A Human Bone Marrow 3D Model to Investigate the Dynamics and Interactions Between Resident Cells in Physiological or Tumoral Contexts

The medullary niche is a complex ecosystem that essential to maintain homeostasis for resident cells. Indeed, the bone marrow, which includes extracellular matrix and various cell types, such as mesenchymal stem cells, osteoblasts, endothelial deeply involved in hematopoietic regulation through direct cell-cell interactions, well cytokine production. To closely mimic this vivo structure conduct experiments reflecting responses of human several 3D models have been created based on biomaterials, relying primarily primary stromal Here, protocol described obtain minimal standardized system easy set up provides features marrow-like structure, combines different populations including reflects heterogeneity marrow tissue. This structure-assembled using calcium phosphate-based particles lines, representative microenvironment-allows monitoring wide variety biological processes by combining or replacing within system. final structures can then either be harvested image analysis after fixation, paraffin-embedding, histological/immunohistochemical staining localization system, dissociated collect each cellular component molecular functional characterization.